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Fig. 4. Presence of actin-binding proteins in RLS. (A) Gelsolin (a) and cofilin (b) organization in ${alpha}$ -SMA-EGFP transfected cells in control conditions. Overlay images with ${alpha}$ -SMA-EGFP show that gelsolin (a, red) completely colocalized with ${alpha}$ -SMA-EGFP (green) in stress fibers, whereas cofilin (b, red) was additionally found at the periphery of the cell (arrow). (B) Cells were treated for 30 minutes with SMA-FP, fixed with 1% paraformaldehyde for 10 minutes followed by methanol for 3 minutes and stained with a large panel of anti actin-binding protein antibodies (see table 1). EGFP fluorescence (green) and immunofluorescence staining (red) with anti-gelsolin (b) and anti-cofilin (e) showed that they were both present in RLS (yellow RLS in the overlay images c and f), whereas many other actin binding proteins ( ${alpha}$ -actinin, h shown as example) were not found (green RLS in the overlay image i). Bar, 10 µm.

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