|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 2. HEK293 cells transfected with ABCG2-K86M display no transport activity. The cells were incubated for 30 minutes at 37°C (accumulation) with 100 nM BODIPY-prazosin in the presence or absence of the specific ABCG2 inhibitor Fumitremorgin C (FTC) (5 µM). The cells were washed and incubated with or without 5 µM FTC for 60 minutes at 37°C (efflux). The histograms show intracellular fluorescence after accumulation and efflux of BODIPY-prazosin (100 nM) in the presence (broken line) or absence (unbroken line) of 5 µM FTC. Fluorescence was measured (FL-1) after excitation with a 488 nm argon laser.
|
