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Fig. 3. ABCG2-wt and ABCG2-K86M co-immunoprecipitate. Total cell lysates from stably transfected HEK293 cells were immunoprecipitated (IP) with anti-MYC antibody and protein G agarose. All cell lysates contained 5 mM N-Ethylmaleimide to inhibit spontaneous cysteine oxidation. The eluate was analyzed by immunoblotting (IB) with either anti-HA or anti-MYC (cmyc) antibodies. Cell lysates were loaded on SDS-PAGE as positive controls for expression of both constructs (lane 7, 8). Singly transfected cells were used as negative controls (lane 3, 4).





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