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Fig. 8. The ABCG2-K86M mutant resides in the ER. Immunocytochemistry co-stainings were performed using an Anti-ABCG2 antibody and antibodies directed against different cellular organelles. Left-hand column, ABCG2 staining; middle column, organelle staining; and right-hand column, overlay of the single stainings. (A,B) Anti-Calnexin (ER), (C,D) Giantin (cis-Golgi) and (E,F) Rab5 (early endosomes). Secondary antibodies were Alexa 488 and Alexa 568. The pictures were obtained by confocal laser-scanning microscopy on a Zeiss LSM 510 confocal microscope.





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