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Fig. 2. Timing of the onset of glycogen accumulation in developing XY gonads and its accumulation in the 11.5-dpc XX male gonad of sex-reversal Sry transgenic mice. (A) Temporal patterns of PAS reactions (red staining; transverse sections) and immunostaining against SOX9 protein (brown staining; sagittal sections) in the XX (left) and XY (middle and right) gonads during early phases of sex differentiation. Similar to the temporal pattern of SOX9 expression, PAS-positive cells are first detected in the XY gonad isolated at 14 ts (arrow for PAS; arrowheads for SOX9). These cells rapidly increased in number, and then aggregated into cord-like structures at 18 ts. No PAS-positive cells are detected in XX gonads at any stages examined. Embryos at approximately 11.0 and 11.5 dpc show 12 and 18 ts, respectively. (B) Transverse sections of the 11.5 dpc (18 ts) embryos of XX wildtype, XY wildtype and XX Sry trangenic embryos stained with PAS. PAS reactions are seen in XY wildtype gonads, as well as in the XX gonads of male embryos carrying the Sry transgene. ms, mesonephros. Bar, 50 µm.

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