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Fig. 5. Glycogen accumulation occurs in XY gonads with severely reduced germ cells that are experimentally induced by busulfan treatment. Sagittal sections showing the effects of germ cell loss on PAS-staining pattern in XY gonads at 11.5 (18 ts; upper plates) and 12.5 (30 ts; lower plates) dpc. PAS staining. No germ cells are detected in these sections of busulfan-treated XY gonads shown in plates B and D. In XY gonads treated with busulfan, PAS-positive cells are properly aggregated into slender cord-like structures (B) similar to those in control XY gonads at the same stage (A), despite the drastic reduction in number of germ cells. In the testes at 12.5 dpc, the testicular cords are formed in the differentiated testes without germ cells, and Sertoli cells show positive PAS staining in both control (C) and busulfan-treated (D) testes. asterisk, germ cell; ms, mesonephros. Bar, 50 µm.





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