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Fig. 7. Testis-specific glycogen accumulation does not require adjacent mesonephros in vitro. Sagittal sections of genital ridges (gonad + mesonephros; A,B,D,E) and genital ridges without adjacent mesonephros (gonad alone; C,F) isolated at 14 ts. PAS staining. Plates show the genital ridge after being cultured for 0 (A,D) or 24 (B,C,E,F) hours. In the 24-hour cultures, glycogen accumulation is induced in XY explants (A,B), but not in XX explants (D,E), of genital ridges. Similar to the pattern of glycogen accumulation in genital ridge cultures, PAS-positive cells are observed in XY explants without mesonephros (C), whereas no PAS-positive cells are detected in XX explants without mesonephros (F). ms, mesonephros. Bar, 50 µm.





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