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Fig. 8. Inhibition of PI3K inhibitor (+LY), but not MEK inhibitor (+PD), on testis-specific glycogen accumulation in developing XY genital ridges in vitro. Sagittal sections of genital ridges (10-11 ts) cultured in the absence (A,D) or presence of MEK inhibitor (50 µM, PD98059; B,E) or PI3K inhibitor (15 µM; LY294002; C,F) with 10% horse serum-DMEM for 48 hours and stained with PAS. In XY explants, the addition of LY294002 to the medium drastically reduced glycogen accumulation in the gonadal area of XY explants (C). By contrast, the MEK inhibitor PD98059 did not exert any obvious effect on glycogen accumulation in XY explants (B). Moreover, no appreciable glycogen accumulation is observed in XX genital ridges treated with these inhibitors (D-F). ms, mesonephros. Bar, 50 µm.
