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Fig. 2. Sas2p and Sas5p interacted with the importins Pse1p and Kap123p. (A) Domains in Pse1p. The blue bar indicates the importin ß homology domain containing an Armadillo repeat (ARM, red). The C-terminal fragments of Pse1p interacting with Sas5p in the two-hybrid assay are illustrated by black lines. (B) Coimmunoprecipitation analysis of Sas5p-myc9/Pse1p-HA3, Sas5p-myc9/Kap123p-HA3, myc6-Sas2p/Pse1p-HA3 and myc6-Sas2p/Kap123p-HA3 interactions. Bound proteins were separated on 12.5% SDS polyacrylamide gels, immunoblotted and probed with an {alpha}-HA antibody. Pse1p-HA3 coimmunoprecipitated with Sas5p-myc9 or myc6-Sas2p (upper panels), and Kap123p-HA3 co-precipitated with Sas5p-myc9 or myc6-Sas2p (lower panels).





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