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Fig. 6. Size fractionation of wild-type and pse1-1 protein extracts prepared from cells (AEY2956 or AEY2957) grown at 24°C and expressing HA-Sas5p (pAE625). The elution profiles of HA-Sas5p were analyzed by gel electrophoresis on 12.5% SDS polyacrylamide gels and immunoblotting of the indicated fractions using an anti-HA antibody. The elution peaks of marker proteins are labeled above.





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