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Fig. 2. Efficient degradation of CPY* depends on the position of N-glycans. (A) Unglycosylated CPY* is stabilized in wild-type cells. CPY* and CPY*0000 were expressed in a {Delta}prc1 strain under identical conditions. Following pulse-chase, radiolabeled proteins were immunoprecipitated using anti-CPY antiserum. The graph represents the mean of three independent experiments. (B) Pulse-chase analysis of CPY* variants carrying only one N-glycan expressed in a {Delta}prc1 strain. Graphs represent the mean of three independent experiments. A representative autoradiogram is shown for each mutant.





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