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Fig. 3. Position rather than number of N-glycans determines the rate of CPY* degradation. Pulse-chase analysis of hypoglycosylated CPY* variants expressed in {Delta}prc1 containing three (A) or two (B) carbohydrate chains shows that only variants lacking the Asn368 glycan (CPY*1100 and CPY*1110) are affected. Graphs represent average data from three independent experiments. (C) A representative autoradiogram is shown for each mutant.





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