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Fig. 4. Htm1p functions via the N-glycans of CPY* but it does not exhibit positional preference. (A) Pulse-chase analysis of CPY* and unglycosylated CPY*0000 expressed in isogenic wild-type (HTM1) and ${Delta}$ htm1 ${Delta}$ prc1 ( ${Delta}$ htm1) strains. (B) Degradation of four hypoglycosylated CPY* species (CPY*0001, CPY*0011, CPY*1100 and CPY*1110) expressed in wild-type (HTM1) and ${Delta}$ htm1 ${Delta}$ prc1 ( ${Delta}$ htm1) strains was analysed by pulse labeling and immunoprecipitation using anti-CPY antibodies. A representative autoradiogram is shown for CPY* and CPY*0000.

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