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Fig. 4. Images of heteromeric connexin formations in NEB1 keratinocytes. Cells were co-transfected with different pairs of wild-type connexins (Cx) fused with ECFP or EYFP. Left-hand panels, Cx-ECFP images, middle panels, Cx-EYFP images and right-hand panels, merged images. Co-transfection with different combinations of (wt)Cx26, (wt)Cx30 and (wt)Cx31 clearly show large gap junction-like aggregates of the fusion proteins at the cell-cell interface. In contrast, none of the combinations with (wt)Cx30.3 showed any aggregates at the plasma membrane. Bar, 10 µm.





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