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Fig. 5. Images of localisation patterns of mutant (D50N)Cx26 and wild-type connexins in co-transfected NEB1 keratinocytes. Cells were co-transfected with a combination of two constructs at a ratio of 1:1. Unlike (D50N)Cx26 on its own, any combination of (D50N)Cx26 with wild-type connexin produced a punctate pattern of localisation in the cytoplasm. Left-hand panels are Cx-ECFP images, middle panels Cx-EYFP images and right-hand panels merged images of Cx-ECFP and Cx-EYFP. Bar, 10 µm.





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