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Fig. 8. Immunoprecipitation analysis. Cells were transfected with a Cx-EGFP construct (first two lanes) or pairs of Cx-EGFP constructs immunoprecipitated with either Cx26 or Cx30.3 antibodies. Western blotting was performed using an anti-EGFP-HRP antibody to detect any Cx-EGFP chimeric protein present. The Cx26 antibody immunoprecipitated Cx26-EGFP+Cx30-EGFP or Cx26-EGFP+Cx30.3-EGFP. In addition, the Cx30.3 antibody immunoprecipitated Cx26-EGFP+Cx30.3-EGFP. The size of the Cx26-EGFP band is ~53 kDa and both Cx30-EGFP and Cx30.3-EGFP were ~56 kDa. The interaction between Cx26 and Cx30 appears to be much stronger although this may be due to the higher levels of Cx26 and Cx30 expression compared to Cx30.3 seen in these experiments.





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