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Fig. 2. Cell surface binding interference experiments between ALCAM/CD166 ligands and the I/F8 scFv, measured by flow cytometry on N592 SCLC cells. Ligand binding in the presence of I/F8 is indicated by black triangles and binding in the presence of the irrelevant anti-NIP scFv is indicated by empty triangles. CD6/Fc and ALCAM/Fc chimeras were used at 10 and 100 nM respectively.





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