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Fig. 6. Ligand-triggered internalization of the ALCAM/CD166 molecule is maximal at the dividing cell contact region. Confocal microscopic imaging of A2774 cells stained with I/F8 plus anti-FLAG mAb, kept on ice (A,D) or allowed to internalize for 40 minutes (B,C), acid-washed to strip the surface if required (B,C,D), fixed, permeabilized and stained with FITC-labeled secondary antibody. Nuclei were counterstained with Propidium Iodide (original magnification 600x).





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