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Fig. 10. Modulation of Ena/VASP activity affects the structure of InlB-mediated phagocytic cups. Vero cells, non transfected (NT) or transiently transfected with Mena-GFP, FPPPP-mito-GFP or APPPP-mito-GFP, were incubated with InlB-coated beads. In the merged images, F-actin is green, GFP is red, and InlB-beads or bacteria are blue. In cells overexpressing Mena (column 2), phagocytic cups around InlB-coated beads (in blue) present a thicker actin network (large arrow) compared to controls (actin cups in non-transfected cells; column 1). Conversely, in cells in which Ena/VASP is sequestered at the mitochondria (FPPPP-Mito, column 3), phagocytic cups seem to contain a less dense actin meshwork (thin arrows) when compared to controls (non-transfected cells, NT, column 1 and APPPP-Mito, column 4). Bar, 2 µm.





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