|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 2. WAVE2-GFP recruitment during InlB-mediated internalisation and ruffling in Vero cells. (A) Recruitment of WAVE2-GFP at InlB-induced ruffles. Vero cells, transiently transfected with WAVE2-GFP, untreated (resting cells) or stimulated with 4.5 nM InlB (+InlB) and stained with phalloidin-546 to detect F-actin. (B) Recruitment of WAVE2-GFP at bacteria-induced phagocytic cups at different stages of the internalisation process. Vero cells were transiently transfected with WAVE2-GFP or N-WASP-GFP. Total bacteria associated with cells were visualized in phase contrast and bacteria that are fully or partly extracellular were identified by labelling with anti-Listeria antibodies prior to cell permeabilisation. F-actin was detected with phalloidin-546. In the merged images (right-hand panels), extracellular bacteria are blue, F-actin is green and the GFP-tagged proteins are red. a, an extracellular bacterium not associated with WAVE-GFP; b, an extracellular bacterium associated with WAVE2-GFP and low amounts of actin filaments; c, an extracellular bacterium; d, an intracellular bacterium in an F-actin-rich phagocytic cup to which WAVE2-GFP localizes. N-WASP-GFP is not recruited to bacteria in F-actin phagocytic cups. Bar, 10 µm (A) and 2 µm (B).
|
