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Fig. 5. WAVE proteins, N-WASP, Abi-1 and VASP siRNA knockdown block Listeria entry into HeLa cells. (A) Silencing of WASP-related proteins and VASP by siRNA. Suppression of gene expression was analysed in control HeLa or in WAVE2-knockdown or Abi1-knockdown cells, by immunoblotting with antibodies against WAVEs isoforms, N-WASP, VASP or actin. (B) Invasion assays in siRNA-transfected cells. The percentage of intracellular bacteria were quantified by gentamicin assays in cells, non-transfected (NT) or transiently transfected, or co-transfected, with the indicated siRNA. The percentage in non-transfected cells was assigned a value of 100. The level of entry in transfected cells is given as a relative value. Values are the mean±s.d. of at least three independent experiments. CT, control siRNA; NW, N-WASP; Rd, random siRNA; W1, WAVE1; W2, WAVE2.





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