spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 1. Microchimeric fetal cells in female thyroid express cytokeratin. Photomicrographs show FISH studies using Cy3-labeled X (orange) and FITC-labeled Y (green) chromosome probes, and immunofluorescence staining with anti-cytokeratin antibody, Texas Red (red). Nuclei are counterstained with DAPI (blue). (A) Male microchimeric cell in maternal thyroid with one Y chromosome (small arrow) and one X chromosome; 1000x magnification. (B) Same cell stained with anti-cytokeratin antibody (large arrow, red), indicating an epithelial cell; 1000x magnification. (C) Interphase FISH study of female thyroid tissue showing a group of male microchimeric cells; 400x magnification. Arrow shows one X and one Y chromosome. This group of cells did not stain positively for cytokeratin; therefore, they are not epithelial cells. (D) Combined FISH and immunofluorescence staining of a group of male microchimeric cells with one X and one Y (small arrow) chromosome. Note that in this plane of focus not all of the X chromosomes can be seen. This group of cells expresses cytokeratin (large arrow); 400x magnification.





Right arrow Return to article