|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 3. The C-terminal domain of ninein displaces endogenous ninein from the centrosome. L929 cells transfected with the GFP/Cter-ninein construct for 6 hours (A) showing a decrease in endogenous ninein at centrioles (arrowhead) compared with control cells (arrow), as judged by staining with an anti-ninein antibody. (B) Distribution of ninein labelling relative intensity at centrosomes in non-expressing cells (white bars), cells 2 hours after transfection (grey bars) and 6 hours after transfection (black bars). Expression of Cter-ninein resulted in a reduction in the level of endogenous ninein at the centrosome, on average, decreases of 42% at 2 hours and of 62% at 6 hours were observed (P<0.001 in both cases). Bars, 10 µm (4 x magnification in insets).
|
