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Fig. 5. Effect of the displacement of ninein on the localization of ${gamma}$ -TuRC components or ${gamma}$ -TuRC docking proteins. L929 (A,B) or Hela (C) cells transfected with GFP/Cter-ninein for 2 hours were fixed and labelled with different markers for ${gamma}$ -TuRC components (A) or for ${gamma}$ -TuRC docking proteins (B,C). (A) ${gamma}$ -Tubulin (a) and Spc98p (b) were displaced from the centrosome in expressing cells (arrowhead) compared with control cells (arrow). (B) Nlp (a) and pericentrin (b) were correctly localized to the centrosome in expressing cells (arrowhead) compared with control cells (arrow). (C) AKAP 450 was also present at the centrosome in expressing cells (arrowhead). Bars, 10 µm (the centrosome areas were magnified four times in insets).

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