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Fig. 7. The ninein N-terminus/C-terminus chimerical fusion displaces ninein but not ${gamma}$ -tubulin from the centrosome. (A) L929 cells expressing GFP Nter/Cter-ninein for 6 hours were fixed and immunolabelled with an anti- ${gamma}$ -tubulin antibody (a), showing that, in expressing cells (arrowhead), ${gamma}$ -tubulin was not displaced from the centrosome compared with control cells (arrow). (b) Quantification of endogenous ${gamma}$ -tubulin labelling relative intensity at the centrosome in expressing (GFP/Nter/Cter-ninein 6 hours, black bars) and non-expressing (white bars) cells, corresponding to the experiment illustrated in a. (B) L929 cells expressing GFP/Nter/Cter-ninein for 6 hours were fixed and immunolabelled with an anti-ninein antibody (a), showing that, in expressing cells (arrowhead), ninein was displaced from the centrosome compared with control cells (arrow). (b) Quantification of endogenous ninein labelling relative intensity at the centrosome, in expressing (GFP/Nter/Cter-ninein 6 hours, black bars) and non-expressing (white bars) cells, corresponding to the experiment illustrated in a. In expressing cells, the relative intensity of ninein label decreased on average to 49% (P<0.001). Bars, 10 µm (4 x magnification in insets).

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