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Fig. 8. The ninein N-terminus/C-terminus chimerical fusion impairs microtubule anchoring but not microtubule nucleation. HCT116 cells transfected with GFP/Nter/Cter-ninein for 5 hours were further treated with nocodazole for 1 hour and analysed, using immunolabelling with anti-{alpha}-tubulin antibody to visualize microtubules upon regrowth. (A) One minute after nocodazole washout, expressing cells (a, arrowhead) formed asters comparable to those in control cells (arrow). (b) Quantification of microtubules and aster formation 1 minute after nocodazole washout, corresponding to the experiment illustrated in a. (B) Ten minutes after nocodazole washout, expressing cells (a, arrowhead) had no asters compared with control cells (arrow). (b) Quantification of microtubules and aster formation 10 minutes after nocodazole washout, corresponding to the experiment depicted in a. Bars, 10 µm.





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