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Fig. 1. Production of HSP60 in the mitochondria of HUVECs fixed with ice-cold acetone/methanol. The nuclei appear red after staining with the fluorescent DNA dye TOPRO-3 and excitation at 543 nm. HSP60 appears green after labelling with a FITC-conjugated monoclonal anti-HSP60 antibody and excitation at 488 nm. (a) Unstressed control cells. (b) Cells exposed to heat stress (42°C, 30 minutes). (c) Detailed view of mitochondrial localization of HSP60 in heat stressed cells.





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