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Fig. 2. CLSM images of HSP60 on the surface of living HUVECs after incubation with the FITC-conjugated monoclonal anti-HSP60 antibody II-13. All images are focused on the top of the cells. (a) Heat-stressed (42°C, 30 minutes) cell showing HSP60 on the cell surface (green regions marked by arrows). The insert shows another heat stressed cell expressing HSP60 on the cell surface. (b) Unstressed control cells with almost no surface expression of HSP60.