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Fig. 6. Force spectroscopy of AbII-13-coated tip on a protein layer of isolated HSP60. HSP60 was electrostatically adsorbed onto mica. (a) Topography image of HSP60 on mica. Single proteins of about 3 nm in height and small aggregates cover the surface of the 500 x 500 nm large area almost completely. The false colour bar at the bottom of the image (0 to 5 nm, from dark to bright) indicates the heights of the objects. (b) Probability density function (pdf) of specific molecular forces giving the distribution of unbinding forces fu. (c) Binding probabilities of AbII-13-coated tips on isolated HSP60 without (left) and with free AbII-13 in solution (Block), proving the specificity of the measured molecular intersections.





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