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Fig. 1. Genomic methylation assays for repetitive sequences. Southern blots of total DNA extracted from wild-type (J1), rescued B9 cells (Dnmt1C/C cells containing a Dnmt1+ BAC) and Dnmt1C/C ES cells, cut with HpaII (methyl sensitive) and MspI (methyl insensitive) isoschizomeric restriction enzymes. (A) Southern analysis of equally loaded DNA using a probe that recognizes minor centromere repeats. (B) Identical Southern blot probed with pKS13b, which recognizes open reading frames of Line-1 repetitive transposable elements. (C) Identical Southern blot probed with mouse CoT1 DNA, which recognizes all highly repetitive sequences in the mouse genome.





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