spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 2. Septins, but not Bnr1p, contribute to shaping the base of the bud. Strains DLY4035 (GAL1p-SWE1), DLY7452 (cdc12-6 GAL1p-SWE1) and DLY6136 (bnr1{Delta} GAL1p-SWE1) were grown to stationary phase in raffinose-containing medium at 24°C, stained with FITC-ConA, washed, and inoculated into galactose-containing medium (to induce Swe1p expression) at 37°C (to inactivate septins). Bud morphology was documented 5 hours later by DIC microscopy, and scored for new buds (unlabeled with FITC-ConA). (A) Image showing the measurements of bud length, neck diameter and bud diameter at 1 µm and 2 µm from the neck. (B) Plots of neck diameter versus bud length for wild-type (DLY4035; CDC12) and septin mutant (DLY7452, cdc12-6) buds. Each circle represents one cell (n=200). (C) Quantitation of neck diameter and bud diameter at 1 µm and 2 µm from the neck. Values are mean ± standard deviation (n=130 for the wild-type, n=184 for the cdc12-6 and n=125 for the bnr1{Delta} strains) Asterisk indicates that the bud diameter difference between cdc12-6 and wild-type cells was statistically significant (P<0.001).





Right arrow Return to article