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Fig. 3. Swe1p-dependent G2 delay promotes assembly of ectopic septin rings. (A,B) Strains DLY4037 (cdc42V36T,K94E), DLY1028 (swe1{Delta}) and DLY4136 (cdc42V36T,K94E swe1{Delta}) were grown to exponential phase in YEPD at 30°C and processed to visualize septin distribution. The proportion of budded cdc42 or cdc42 swe1 cells displaying ectopic septins in the bud (top graph: mislocalized septins) or defective (faint, patchy, or absent) septins at the neck (bottom graph: misorganized septins) were scored. Representative cells (B) show that septins at the neck are still defective in cdc42V36T,K94E cells that lack Swe1p. (C) Strains RSY136 (GAL1p-SWE1), DLY4141 (cdc42V36T,K94E GAL1p-SWE1), and DLY4838 (cdc12-6 GAL1p-SWE1) were grown to exponential phase in sucrose-containing medium at 24°C, induced to overexpress Swe1p by addition of galactose (2% final concentration), and processed to visualize septin distribution or cell morphology after a further 4 hours at 24°C. Bar, 10 µm.





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