JCS01728 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1 (a) BrdU assay was performed as described in Materials and Methods by assessing the percentage of BRDU-positive 2fTGH, U3A or U3A-ST1 cells by FACScan flow cytometer following irradiation. (b) FACScan data showing staining with propidium iodide and antibody against phosphorylated histone H3, and the percentage of M-phase cells in 2fTGH, U3A or U3A-ST1 cells following 4 hours of 10 Gy g-irradiation.

• Supplemental Figure 2 -

  Fig. S2 HCT15 cells (Chk2-deficient) and HCT15 cells stably expressing wild-type HA-tagged Chk2 (Chk2wt-HA) (a) or kinase-dead HA-tagged Chk2 (Chk2kd-HA) (b) were immunoprecipitated with HA and then immunoblotted for STAT-1 (ST1) or HA.