spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 3. Modulation of 53BP1 and MDC1 levels by STAT-1. (A) Cell lysates from 2fTGH, U3A and U3A-ST1 cells were immunoblotted for MDC1 and 53BP1. 53BP1 and MDC1 expression is reduced in STAT-1-deficient U3A cells. (B) RT-PCR showing reduced 53BP1 and MDC1 mRNA levels in U3A cells. MDC1 and 53BP1 mRNA levels were assessed from 2fTGH, U3A or U3A-ST1 cells. (C) 2fTGH, U3A or U3A-ST1 cells were exposed to {gamma}-IR (2 Gy), fixed after 2 hours and stained with anti-53BP1 or anti-MDC1 antibody. The dimensions of the field of view are 40 mM x40 mM. (D,E) The MDC1 promoter is modulated by STAT-1. The MDC1-reporter construct was transfected into STAT-1+/+ and STAT-1–/– MEF cells, and U3A and U3A-ST1 cells together with either full length STAT-1 (ST1), STAT-1ß (ST1B) or a control vector. Upper panels in D and E show immunoblots of transfected cells for STAT-1{alpha} (ST1{alpha}) or STAT-1ß (ST1ß).





Right arrow Return to article