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Fig. 2. Biosynthetic trafficking of transiently transfected T7-Syt IX and Flag-Syt IX-GFP in RBL cells. RBL cells, transiently transfected with Flag-Syt IX-GFP (a-f) or T7-Syt IX (g-l) cDNA were grown on glass coverslips. Two hours post transfection, cells were placed for 2 hours at 19° and subsequently shifted to 37°C (time zero) for the indicated time periods, in the absence or presence of BFA (5 µg/ml) for the last 30 minutes of incubation at 37°C. Cells were subsequently labeled with polyclonal anti-Syt IX C2A antibodies followed by Cy3-conjugated donkey anti-rabbit IgG (g-l). Bars, 3 µm.
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