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Fig. 6. PKC-mediated phosphorylation is crucial for ERC localization of Syt IX. (A) RBL cells stably transfected with T7-Syt IX cDNA were grown on glass coverslips. Cells were left untreated or incubated with TPA (50 nM) for 30 minutes at 37°C prior to labeling with polyclonal anti-Syt IX C2A antibodies followed by Cy3-conjugated donkey anti-rabbit IgG. Bars, 3 µm. (B) RBL cells, transiently transfected with T7-Syt IX or Flag-Syt IX-GFP cDNA were grown on glass coverslips for 3 hours. Cells were then either left untreated or incubated with Go 6976 (100 nM) for additional 15 hours. Cells were subsequently labeled with polyclonal anti-Syt IX C2A antibodies followed by Cy3-conjugated donkey anti-rabbit IgG. Bars, 3 µm.
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