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Fig. 7. The slower-migrating form of Syt IX resides exclusively at the ERC. (A) Cell extracts (80 µg) derived from untreated or TPA (50 nM, 30 minutes)-treated Flag-Syt IX-GFP-expressing cells were resolved by SDS-PAGE and subjected to immunoblotting with anti-Syt IX C2A antibodies. (B) Cell homogenates derived from RBL cells stably transfected with T7-Syt IX or Flag-Syt IX-GFP cDNA were fractionated on continuous sucrose gradients as described under Materials and Methods. Fractions were collected from the top, subjected to SDS-PAGE and immunoblotted with anti-Syt IX C2A (a) or anti-GFP (b) antibodies.





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