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Fig. 8. Localization of Syt IX in CHO cells. CHO cells stably transfected with Syt IX (a) or Flag-Syt IX-GFP (b,c) cDNA were grown on glass coverslips in the absence or presence of TPA (50 nM) as indicated. Cells were subsequently labeled with polyclonal anti-Syt IX C2A antibodies followed by Cy3-conjugated donkey anti-rabbit IgG (a). Bars, 3 µm.





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