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Fig. 5. The Euglena pSSU pre-sequence region between the signal peptidase cleavage site and stop-transfer membrane anchor sequence functions as a transit peptide with pea chloroplasts. Schematic diagram (left) of pSSU deletion constructs indicating the pre-sequence (PRESEQ), hydrophobic domains (dark boxes), predicted signal peptidase cleavage site (arrow) and higher plant stromal processing peptidase processing site (arrow) respectively. In vitro synthesized ³⁵S-labeled proteins (tran) corresponding to a one-unit pSSU encoded by plasmid 1pSSU (A) and pSSUs with pre-sequence deletions encoded by plasmids 1PSSU1-111 (B), 1pSSU289-342 (C), 1pSSU289-402 (D) and mSSU (E) were incubated with Euglena chloroplasts with the indicated additions (+) and omissions (–). Fluorographs (right) of ³⁵S-labeled protein from Percoll-purified chloroplasts incubated with or without thermolysin show protease-protected proteins absent from the translation products (arrows) and present in the translation products (arrowheads) were recovered with chloroplasts incubated with the pSSUs lacking the pre-sequence signal peptide domain (panel B), lacking the signal peptide domain and the hydrophobic stop-transfer membrane anchor sequence (panel C) and lacking the signal peptide domain and the sequence C-terminal to the start of the hydrophobic stop-transfer membrane anchor sequence (panel D) localizing the functional transit peptide to the region between the signal peptidase cleavage site and the stop-transfer membrane anchor sequence.

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