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Fig. 6. Euglena preproteins are not post-translationally imported into Euglena chloroplasts. Schematic diagram (left) of pSSU deletion constructs indicating the pre-sequence, (PRESEQ) hydrophobic domains (dark boxes), predicted signal peptidase cleavage site (arrow) and higher plant stromal processing peptidase processing site (arrow) respectively. In vitro synthesized [35S]-labeled proteins (tran) corresponding to a one-unit pSSU encoded by plasmid 1pSSU (A) and pSSUs with pre-sequence deletions encoded by plasmids 1PSSU{Delta}1-111 (B), 1pSSU{Delta}289-342 (C), 1pSSU{Delta}289-402 (D) and mSSU (E) were incubated with Euglena chloroplasts with the indicated additions (+) and omissions (–). Fluorographs (right) of 35S-labeled protein from Percoll-purified chloroplasts incubated with or without thermolysin show that pSSU and pSSUs with pre-sequence deletions but not mature SSU can associate with Euglena chloroplasts but can not be imported post-translationally.





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