spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 1. Activation of ERK5 leads to disruption of the actin cytoskeleton. (A) MEK5DD activates ERK5 but not ERK1/2. Expression vectors for HA-tagged ERK5 and Flag-tagged ERK2 were co-transfected with expression plasmids for HA-MEK5DD, Myc-H-RasV12 or empty vector. After 24 hours cells were lysed and analysed by SDS-PAGE followed by western blotting for epitope tags, ERK5 or phospho-ERK. The two top arrows indicate the slower migrating phosphorylated form of ERK5 (phosphorylated) and non-phosphorylated ERK5. (B,C) NIH3T3 cells were microinjected, in the presence of serum, with expression vectors for activated MEK1 (MEK1EE) and ERK2, activated MEK5 (MEK5DD) and ERK5. 3 hours post-injection, cells were starved of serum and after 24 hours, plates were fixed and stained for polymerised actin with Texas Red-phalloidin and for the expressing proteins with antibodies against MEK1, ERK2, MEK5 and ERK5. Arrows indicate microinjected cells. Bar, 20 µm.





Right arrow Return to article