JCS02305 Supplementary Material

Files in this Data Supplement:

• Supplemental Table (Adobe PDF)
• Supplemental Figure 1 -

  Fig. S1. Kymographs constructed from GFP-tubulin films showing normal dynamics within a microtubule bundle in wild-type cells and uncoordinated behaviour of microtubules within a bundle in amo1D cells. White arrowheads indicate periods of coordination of shrinkage and growth of microtubules in wild-type cells. Black arrowheads indicate microtubules within one bundle touching the cell tip at the same time, that later depolymerise sequentially in amo1D cells.

• Movie 1 -

  Movie 1. Time-lapse microscopy of live wild-type cells expressing Cut11-GFP (nuclear envelope) and GFP-Atb2 (microtubules). The nuclear envelope suffers deformations as microtubules attached to it hit the cell cortex and push against it.

• Movie 2 -

  Movie 2. Time-lapse microscopy of live amo1D cells expressing Cut11-GFP (nuclear envelope) and GFP-Atb2 (microtubules). The nuclear envelope suffers deformations as tangential microtubules hit the cell cortex and push against it, indicating that microtubule attachment to the nuclear membrane is not impaired in amo1D cells.