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Fig. 3. Microtubular defects in amo1{Delta} cells. (A) Tubulin immunofluorescence of cold and MBC treated cells at time 0 (t0) and 1 minute (1') after allowing microtubule repolymerisation. (B,C) Time-lapse series of GFP-tubulin in live wild-type (B) and amo1{Delta} cells (C) showing uncoordinated growth and shrinkage in amo1{Delta} cells. Numbers indicate time in seconds. Arrowheads in B indicate polymerising microtubules (white) and depolymerising microtubules (yellow) in the same bundle. White arrowheads in C indicate polymerising microtubules and yellow arrowheads depolymerising microtubules in bundles with more than one microtubule touching the cell tip at the same time. (D) Tea1 (green) and tubulin (red) immunofluorescence in wild-type and amo1{Delta} cells. Arrowhead depicts abnormal accumulation of Tea1. Bar, 10 µm.





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