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Fig. 2. ABCG2 immunolocalization in the ocular surface. A. Whole human limbus. To generate this micrograph, multiple frames were spliced together. Note that ABCG2 is intensively expressed in the recedes of the Palisades of Vogt (Pal. Vogt), in particular near the conjunctiva. The immunostaining pattern suggests a cluster organization. Individual clusters are marked by asterisks. (B) High magnification micrograph of a single Palisade of Vogt. (C) The section adjacent to that stained in B was processed with the omission of the anti-ABCG2 antibody as a control. (D) Human corneal central epithelium. BL, Bowman layer. (E-G) Selected images of the human conjunctiva. ABCG2 expression is extremely low in the very thick palpebral zone (E). It is moderately present in the thinner transition area (F) and at higher levels in the Goblet cell-rich palpebral-fornical zone (G). Asterisks indicate Goblet cells. Magnifications: A,G 120x; B,C,E,F 250x; D 400 x.





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