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Fig. 8. CFDA-SE retention of freshly plated limbal epithelial cells as a function of their SSC. The time elapsed since plating is indicated within each plot. The decrease in CFDA-SE fluorescence primarily reflects cell division. The general population of adherent cells exhibits large decreases in fluorescent intensity in the 24-48, 48-72 and 72-96 hour intervals whereas the great majority of the LSSC cells seem to retain most of the initial fluorescence for the first 72 hours. The experiment shown is representative of two experiments with limbal and two experiments with conjunctival epithelial cells.
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