QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 4. Transcripts for GLG1 and GLG2 in various cells. (a) RT-PCR analysis identifies transcripts for both GLG1 and GLG2 in human peripheral blood monocytes. Total RNA from human monocytes was reverse transcribed with primer 2 (for GLG1) or primer 3 (for GLG2). Reverse transcription reaction mixture (1:100) was used for the amplification of isoform-specific sequences from transmembrane domain to the 3'-UTR sequence of transcripts of GLG1 and GLG2 (b) Cellular specificity of splicing activity for the production of GLG2 transcript. Poly (A)⁺ RNAs from various tumor lines (1, melanoma G361; 2, lung carcinoma A549; 3, colorectal adenocarcinoma SW480; 4, Burkitt's lymphoma Raji; 5, lymphoblastic leukemia MOLT-4; 6, chronic myelogenous leukemia K-562; 7, HeLa cell S3; 8, promyelocytic leukemia HL-60) were analyzed with GLG isoform-specific probes as described in the Materials and Methods. RNA molecular markers for 9.49, 7.46 and 4.40 kb were also shown. (c) Species specificity for the alternative splicing activity. Total RNAs from African Green monkey COS-2 (lane 1), human melanoma cell lines BOWES (lane 2), murine RAW cells (lane 3 and 4), CHO cells (lane 5) and murine SP2/0 (lane 6) were analyzed with the originally cloned 1.8 kb fragment. Data from two different experiments are shown.

Return to article