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Fig. 5 . Stable expression of LEDGF/p75.NLS– in L cells; subcellular localization and interaction with IN. Stable cell lines were derived by puromycin selection. (A) Confocal immunofluorescence with anti-LEDGF/p75 mAb. DAPI staining (right) indicates chromatin (approximately 50% of the cells in this stable L cell line express the LEDGF/p75 mutant). (B) The same cells after transfection of HIV-1 IN. LEDGF/p75.NLS– and IN colocalize (overlay, right).





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