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Fig. 7. C-terminal segments of LEDGF/p75 fused to NLS-GFP: IN interaction. L cells were cotransfected with HIV-1 IN and either NLS-GFP, NLS-GFP/330-417, or NLS-GFP/306-530. shRNA target-site-mutated (siMut) versions of LEDGF/p75 were used in all expression plasmids. (A) Confocal microscopy. (B) Co-immunoprecipitation of NLS-GFP/330-417 and IN. Immunoprecipitation was carried out with a mAb to GFP, followed by immunoblotting for GFP (lower) and IN (upper).





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