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Fig. 1. Meiosis I spindle assembly in a wild-type ncdgfp* oocyte. Images from a time-lapse sequence (Movie 1 in supplementary material) show the steps of spindle assembly in a live oocyte. (A,B) Germinal vesicle breakdown. The margin of the nuclear envelope shows regions of unevenness (arrowhead). The dark spot in the germinal vesicle (arrow) is the karyosome of condensed chromosomes, or endobody. (C,D) Dispersion of the germinal vesicle contents into the ooplasm. The arrows indicate the microtubule foci or asters, one of which is associated with the endobody. (E-G) Nucleation of randomly oriented microtubules at the endobody. (H) Differentiation of the endobody into microtubule-associated bivalent chromosomes. (I) Formation of lateral interactions between microtubule-associated chromosomes. (J-L) Formation and elongation of a bipolar spindle. Time, minutes:seconds. Bar, 5 µm.





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