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Fig. 7. Binding by the NcdNKGFP* motor to meiosis I spindle microtubules. The dissociation rate (kd) of the motor from microtubules was estimated for NcdNKGFP* and wild-type NcdGFP* using fluorescence loss in photobleaching (FLIP) experiments. Plots of fluorescence (grey value) as a function of time (s, seconds) were fitted to a single exponential curve to estimate the kd. Representative plots are shown for NcdNKGFP* (crosses and red line, kd=0.035 second–1) and wild-type NcdGFP* (filled circles and black line, kd=0.069 second–1). The mean kd for NcdNKGFP* was 0.042±0.005 second–1 (n=16) and that for wild-type NcdGFP* was 0.065±0.005 second–1 (n=16). Curves are normalized to a starting grey level of 1.000 for comparison.





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